Development of novel simple sequence repeat markers from ramie (Boehmeria nivea L. Gaudich) and analysis of genetic diversity in its genetic resources

作者：Kim Ho Bang^*; Uhm Yoon Kyung; Kim Jae Joon; Lim Sanghyun; Kim Young Mi; Jung Yong Su; Roh Kyung Hee; Jang Young Seok; Lee Sanghyun; Um Yurry

来源：Horticulture Environment and Biotechnology, 2016, 57(5): 519-528.

DOI：10.1007/s13580-016-0094-9

• fiber crop
• genetic structure
• internal transcribed spacer
• purity test
• taxonomic relationship

Boehmeria nivea (ramie), a subshrub of the Urticaceae family and an important fiber crop, has been grown in Asian countries, including Korea, for many centuries. Traditionally, in some regions of Korea, ramie leaves are also used as a major ingredient in 'Songpyeon' rice cakes. Despite its economic importance, the molecular genetics of ramie have not yet been studied in detail. Researchers from Yeong-Gwang Agricultural Technology Center collected genetic resources of ramie from a variety of local sites in Korea. Nuclear internal transcribed spacer sequences amplified from 90 genetic resources showed no variation in size or sequence between them or B. nivea reference samples, indicating that all the genetic resources could be taxonomically classified as B. nivea. To systematically and efficiently manage these genetic resources, we developed simple sequence repeat (SSR) markers for ramie using the magnetic bead hybridization selection method. Finally, we selected 17 SSR markers that showed possible polymorphism among the genetic resources and analyzed the genetic diversity of ramie collection. These 17 markers detected a total of 140 alleles, ranging from 3 to 17 alleles per accession. The average genetic diversity value was 0.61, ranging from 0.37 to 0.84. Average polymorphism information content was 0.56, ranging from 0.34 to 0.82. Genetic cluster analysis based on SSR data revealed that the genetic collection of ramie plants could be classified into four distinct clusters. A purity test on several of the genetic resources using polymorphic SSR markers revealed that some resources were impure. These newly developed SSR markers might be useful for diverse genetic analysis including the management of genetic collections, establishment of core collections, and cultivar identification.

• 出版日期2016-10