A novel detection strategy for odorant molecules based on controlled bioengineering of rat olfactory receptor 17

作者：Hou Yanxia^*; Jaffrezic Renault Nicole; Martelet Claude; Zhang Aidong; Minic Vidic Jasmina; Gorojankina Tatiana; Persuy Marie Annick; Pajot Augy Edith; Salesse Roland; Akimov Vladimir; Reggiani Lino; Pennetta Cecilia; Alfinito Eleonora; Ruiz Oscar; Gomila Gabriel; Samitier Josep; Errachid Abdelhamid

来源：Biosensors and Bioelectronics, 2007, 22(7): 1550-1555.

DOI：10.1016/j.bios.2006.06.018

• G protein-coupled receptors
• olfactory receptor 17
• self-assembled multilayer
• electrochemical impedance spectroscopy
• biosensor

In this study, we report a dose-dependent detection of odorant molecules in solution by rat olfactory receptor 17 (OR 17) in its membrane fraction. The OR 17 is immobilized on a gold electrode by multilayer bioengineering based on a mixed self-assembled monolayer and biotin/avidin system, which allows for a well-controlled immobilization of the bioreceptor within its lipid environment. The odorant detection is electronically performed in a quantitative manner by electrochemical impedance spectroscopy (EIS) measurements on samples and controls. circle dot 2006 Elsevier B.V.

• 出版日期2007-2-15
• 单位华中师范大学