A novel efficient method for maize genetic transformation: Usage of agrobacterial monolayer

作者：Danilova S A^*; Kusnetsov V V; Dolgikh Yu I

来源：Russian Journal of Plant Physiology, 2009, 56(2): 258-263.

DOI：10.1134/S1021443709020150

• Zea mays
• Agrobacterium tumefaciens
• embryogenic callus
• immature embryos
• regeneration
• transformation
• agrobacterial monolayer
• transgenic plants

A new approach for maize genetic transformation using Agrobacterium tumefaciens was developed. It allows the usage of both immature embryos and embryogenic calluses as explants. This method includes long-term (15-20 days) cultivation of maize tissues on the agrobacterial monolayer. In this case, necrotic lesions did not appear and all cells were alive. Regenerated plants expressed genes for neomycine phosphotransferase and beta-glucuronidase. The presence of the nptII gene was confirmed by PCR analysis and Southern-blot hybridization. In dependence on maize genotype and experimental conditions, the frequency of genetic transformation varied from 1.5 to 16%. Inheritance of genes introduced was demonstrated. The method elaborated could be applied to a wide range of maize genotypes.

• 出版日期2009-3