MitoQ(10) induces adipogenesis and oxidative metabolism in myotube cultures

作者：Nierobisz Lidia S^*; McFarland Douglas C; Mozdziak Paul E

来源：Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 2011, 158(2): 125-131.

DOI：10.1016/j.cbpb.2010.10.003

• MitoQ(10)
• PGC-1 alpha
• PPAR gamma
• Adipocytes
• Skeletal muscle
• Avian

Coenzyme Q(10) (CoQ(10)) plays an essential role in determination of mitochondrial membrane potential and substrate utilization in all metabolically important tissues. The objective of the present study was to investigate the effect of Coenzyme Q analog (MitoQ(10)) on oxidative phenotype and adipogenesis in myotubes derived from fast-glycolytic Pectoralis major (PM) and slow-oxidative Anterior latissimus dorsi (ALD) muscles of the turkey (Meleagris gallopavo). The myotubes were subjected to the following treatments: fusion media alone, fusion media +125 nM MitoQ(10), and 500 nM MitoQ(10). Lipid accumulation was visualized by Oil Red 0 staining and quantified by measuring optical density of extracted lipid at 500 nm. Quantitative Real-Time PCR was utilized to quantify the expression levels of peroxisome proliferator-activated receptor (PPAR gamma) and PPAR gamma co-activator-1 alpha (PGC-1 alpha). MitoQ(10) treatment resulted in the highest (P<0.05) lipid accumulation in PM myotubes. MitoQ(10) up-regulated genes controlling oxidative mitochondrial biogenesis and adipogenesis in PM myotube cultures. In contrast, MitoQ(10) had a limited effect on adipogenesis and down-regulated oxidative metabolism in ALD myotube cultures. Differential response to MitoQ(10) treatment may be dependent on the cellular redox state. MitoQ(10) likely controls a range of metabolic pathways through its differential regulation of gene expression levels in myotubes derived from fast-glycolytic and slow-oxidative muscles. Published by Elsevier Inc.

• 出版日期2011-2