Pacific ciguatoxin 1B-induced modulation of inflammatory mediators in a murine macrophage cell line

作者：Matsui Mariko; Kumar Roine Shilpa; Darius H Taiana; Chinain Mireille; Laurent Dominique; Pauillac Serge^*

来源：Toxicon, 2010, 56(5): 776-784.

DOI：10.1016/j.toxicon.2009.05.039

• Ciguatera Fish Poisoning
• Cytokines
• Nitric oxide
• Neurotoxins
• Inflammatory system
• Quantitative PCR

Ciguatoxins, potent marine neurotoxins responsible for ciguatera, exert their numerous damaging effects through primary binding to the voltage-sensitive sodium channels of excitable cells. Using RAW 264.7 murine macrophages, we report the first experimental study presenting evidence that P-CTX-1B (the most potent congener from the Pacific) could modulate mRNA expression of pro- and anti-inflammatory cytokines as well as of inducible nitric oxide synthase (iNOS). P-CTX-1B, unlike other less potent marine polyether toxins, P-CTX-3C and PbTx-3, induced the overexpression of interleukin (IL)-1 beta, IL-6, IL-10, tumor necrosis factor-alpha and iNOS with different magnitude and kinetic profiles, as compared to bacterial lipopolysaccharide (LPS). Unlike LPS, P-CTX-1B did not modulate IL-11 expression. In this report, we provide new evidence of the P-CTX-1B iNOS- and cytokines-inducing ability and shed new light on host response to potent neurotoxins.

• 出版日期2010-10