A "green" method to detect aflatoxin B-1 residue in plant oil based on a colloidal gold immunochromatographic assay

作者：Sun, Qing; Zhu, Zhu; Deng, Qian-min; Liu, Jie-min; Shi, Guo-qing^*

来源：Analytical Methods, 2016, 8(3): 564-569.

DOI：10.1039/c5ay02779a

• RAPID DETECTION
• OLIVE OIL
• PEANUT OIL
• IMMUNOASSAY
• ANTIBODY
• G(1)
• G(2)

A "green", simple, fast and sensitive method for detecting aflatoxin B-1 (AFB(1)) in plant oil was developed based on a colloidal gold immunochromatographic assay. Anti-AFB(1) antibody-coated gold colloids were used as detection probes for the immunological recognition of AFB(1). Unlike the traditional methods that require use of organic solvents and complex sample pre-treatment processes, the detection of AFB(1) residue in this work was achieved by a "green" process involving simply mixing oil and water in the indicated ratio and then loading the mixture onto the test strip for visual inspection. The visual detection limit of the test strip for AFB(1) in oil was 1.5 mu g kg(-1) and the analysis could be completed in 5 min. Recoveries for AFB(1) in five oil samples were within the range of 88.7-122.3% according to a semiquantitative analysis. The results of the test strip were in good agreement with those of HPLC. This test strip can be applied to the household detection of AFB(1) in plant oil.

• 出版日期2016
• 单位北京科技大学