科研之友
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摘要
Oxalate biosensors (OBs) are based on either measurement of oxygen or electrons generated from splitting of H(2)O(2) or heat released during oxidation of oxalate by immobilized oxalate oxidase. OBs are grouped in four classes based on probe used (DO meter, electrochemical, SIRE technology and thermochip). OBs work optimally at: time, 5 s to 15 min; pH, 2.8 - 8.0; temp., 30- 38 degrees C and linearity, 1.0 - 100 mu M oxalate. OBs are employed to determine oxalate in urine, serum, plasma, foodstuffs and can be reused 80-400 times over a period of 3 days to 4 months. This review presents OBs as well as their merits and demerits.
- 出版日期2010-7
