Computational prediction and analysis of potential antigenic CTL epitopes in Zika virus: A first step towards vaccine development

作者：Dikhit Manas R; Ansari Md Yousuf; Vijaymahantesh; Kalyani; Mansuri Rani; Sahoo Bikash R; Dehury Budheswar; Amit Ajay; Topno Roshan K; Sahoo Ganesh C; Ali Vahab; Bimal Sanjiva; Das Pradeep^*

来源：Infection, Genetics and Evolution, 2016, 45: 187-197.

DOI：10.1016/j.meegid.2016.08.037

• Zika virus
• ZIKV
• Epitope
• Epitope mapping
• CD8+T cell
• MHC class I
• Immunoinformatics

The Zika virus disease is an Aedesmosquito-borne disease caused by the ZIKA virus. The unavailability of vaccines or proper chemotherapeutic treatment emphasizes the need for the development of preventive and therapeutic vaccines. T cell specific epitopes have been used as vaccine candidates to generate desired immune responses against a variety of viral pathogens. Herein, the immune-informatics approach was used for the screening of potential major histocompatibility complex class I restricted epitopes, which may be competent to generate a cellmediated immune response in humans. A total of 63 epitopes were identified, which revealed a comprehensive binding affinity to the 42 different human leukocyte antigen class I supertypes: A01, A02, A08, A23, A24, A25, A26, A29, A30, A32, A66, A68, A69, A80, B07, B08, B14, B15, B27, B35, B39, B40, B42, B45, B46, B48, B51, B53, B54, B57, B58, B83, C12, C03, C04, C05, C06, C07, C08, C12, C14, and C15, andwhich had no homologs in humans. By combining the human leukocyte antigen binding specificity and population coverage, nine promiscuous epitopes located in Capsid 1 Protein (MVLAILAFL(P1)), Envelop Protein (RLKGVSYSL (P2) and RLITANPVI (P3)), NS2A (AILAALTPL (P4)), NS4B (LLVAHYMYL (P5) and LVAHYMYLI (P6)) and NS5 (SLINGVVRL (P7), ALNTFTNLV (P8) and YLSTQVRYL (P9)) were shortlisted. Most of these consensus epitopes revealed 100% conservancy in all Zika virus strains andwere very less conserved against the human proteome. The combination of the selected epitopes accounted for an optimal coverage in the worldwide population (N99%) independent of ethnicity. Structural analysis of these selected epitopes by the PatchDock web server showed their preferential mode of presentation to the T cell receptor. All these results recommended the possibility of a combined epitope vaccine strategy and can therefore be further investigated for their immunological relevance and usefulness as vaccine candidates.

• 出版日期2016-11
• 单位河北医科大学