Assessment of allergenicity and mutagenicity of bar-transgenic rice using a mouse (Mus musculus) model

作者：Liu, Jin; Huang, Yi; Sun, YanBo; Yan, HengMei^*

来源：Journal of Food Agriculture and Environment, 2012, 10(2): 236-240.

• Bar-transgenic
• rice
• PAT enzyme
• allergenicity
• mutagenicity
• Mus musculus

To assess the safety of Bar-transgenic rice, its allergenicity and mutagenicity were tested on 100 Kunming mice (Mus musculus). The test mice were randomly divided into five groups of twenty mice each and were given diets containing varying doses of genetically modified (GM) Bar68-1 rice, D68 (non-GM) rice, and routine feed. On the 180th day, five mice from each group were randomly sampled, and the IgE and DAO levels in their serum and the sIgA in their small intestinal mucus were quantified. The quantification of these chemicals was done for three generations of mice. Moreover, the 12S rDNA and 16S rDNA conserved region of the small intestinal mitochondrial DNA (mtDNA) were sequenced using the double sequencing method. The results indicate no significant difference in the serum sIgA, DAO and IgE between the Bar68-1 GM rice group and the non-GM D68 rice groups (P>0.05). The Bar-transgenic rice did not have allergenic effects on the mice and no mutation was found in the 12S rDNA and 16S rDNA conserved region of the intestinal mtDNA.

• 出版日期2012-4
• 单位湖南师范大学