A molecular probe for Basidiomycota: the spermidine synthase-saccharopine dehydrogenase chimeric gene

作者：Leon Ramirez Claudia G; Valdes Santiago Laura; Campos Gongora Eduardo; Ortiz Castellanos Lucila; Arechiga Carvajal Elva T; Ruiz Herrera Jose^*

来源：FEMS Microbiology Letters, 2010, 312(1): 77-83.

DOI：10.1111/j.1574-6968.2010.02099.x

• Basidiomycota
• spermidine synthase
• saccharopine dehydrogenase
• chimeric gene
• molecular probe

By means of an in silico analysis, we demonstrated that a previously described chimeric gene (Spe-Sdh) encoding spermidine synthase, a key enzyme involved in the synthesis of polyamines, and saccharopine dehydrogenase, an enzyme involved in lysine synthesis in fungi, were present exclusively in members of all Basidiomycota subphyla, but not in any other group of living organisms. We used this feature to design degenerated primers to amplify a specific fragment of the Spe-Sdh gene by PCR, as a tool to unequivocally identify Basidiomycota isolates. The specificity of this procedure was tested using different fungal species. As expected, positive results were obtained only with Basidiomycota species, whereas no amplification was achieved with species belonging to other fungal phyla.

• 出版日期2010-11