A Toolbox for Rapid Quantitative Assessment of Chronological Lifespan and Survival in Saccharomyces cerevisiae

作者：Chadwick Sarah R; Pananos Athanasios D; Di Gregorio Sonja E; Park Anna E; Etedali Zadeh Parnian; Duennwald Martin L; Lajoie Patrick^*

来源：Traffic, 2016, 17(6): 689-703.

DOI：10.1111/tra.12391

• caloric restriction
• cell viability
• chronological lifespan
• computer-assisted analysis
• fluorescence
• heat shock proteins
• yeast

Saccharomyces cerevisiae is a well-established model organism to study the mechanisms of longevity. One of the two aging paradigms studied in yeast is termed chronological lifespan (CLS). CLS is defined by the amount of time non-dividing yeast cells can survive at stationary phase. Here, we propose new approaches that allow rapid and efficient quantification of survival rates in aging yeast cultures using either a fluorescent cell counter or microplate imaging. We have generated a software called ANALYSR (Analytical Algorithm for Yeast Survival Rates) that allows automated and highly reproducible analysis of cell survival in aging yeast cultures using fluorescent data. To demonstrate the efficiency of our new experimental tools, we tested the previously characterized ability of caloric restriction to extend lifespan. Interestingly, we found that this process is independent of the expression of three central yeast heat shock proteins (Hsp26, Hsp42, Hsp104). Finally, our new assay is easily adaptable to other types of toxicity studies. Here, we assessed the toxicity of various concentrations of acetic acid, a known contributor of yeast chronological aging. These assays provide researchers with cost-effective, low-and high-content assays that can serve as an efficient complement to the time-consuming colony forming unit assay usually used in CLS studies.

• 出版日期2016-6