科研之友
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摘要
To improve the detection of ovulation and early diagnosis of pregnancy on dairy farms, a disposable electrochemical immunosensor was developed using indirect competitive immunoassays and screen-printed carbon electrodes. After optimisation of the assay conditions, the linear range was from 0.16 to 50ng/mL. The limit of detection was 0.16ng/mL. There was no cross-reactivity with testosterone, 17--estradiol and estriol. The intra-assay coefficient of variation was less than 13% and interassay coefficient of variation was <9%. The percentage recovery was from 95.8% to 115.7%. Stability lasts 4days at 4 degrees C. The results of preliminary application trials indicate that the method could have practical potential.
