SO2 inhalation induces protein oxidation, DNA-protein crosslinks and apoptosis in rat hippocampus

作者：Sang Nan^*; Hou Li; Yun Yang; Li Guangke

来源：Ecotoxicology and Environmental Safety, 2009, 72(3): 879-884.

DOI：10.1016/j.ecoenv.2008.07.007

• SO2
• Protein oxidation
• DNA-protein crosslinks
• Apoptosis
• Rat hippocampus

Previous studies provide evidence for the possible neurotoxicity Of SO2, but little information is available about its mechanisms. In the present study, SO2 inhalation-induced effects on the protein oxidation, DNA-protein crosslinks and apoptosis in rat hippocampus were studied, by exposing Wistar rats to SO2 at 14, 28 and 56 mg/m(3). The results indicate that the protein carbonyl content, an indicator of protein oxidation, and DNA-protein crosslink coefficient were significantly augmented with concentration-dependent properties. In addition, SO2 inhalation at all concentrations tested caused the increases of caspase-3 activity and number of TUNEL positive staining neuron and the statistical difference was observed after 28 and 56 mg/m(3) exposure, suggesting the occurrence of apoptosis. The results imply that attacking protein, nucleic acids and lipids by free radicals, generated Via SO2 derivatives in vivo, is one of the main mechanisms for SO2-induced injuries in central neuronal system.

• 出版日期2009-3
• 单位山西大学