Molecular Characterization of the Polymicrobial Flora in Chronic Rhinosinusitis

作者：Stephenson Marie France; Mfuna Leandra; Dowd Scot E; Wolcott Randall D; Barbeau Jean; Poisson Michel; James Garth; Desrosiers Martin^*

来源：Journal of Otolaryngology-Head & Neck Surgery, 2010, 39(2): 182-187.

DOI：10.2310/7070.2009.090060

• anaerobic bacteria
• bacterial biofilm
• chronic rhinosinusitis
• endoscopic sinus surgery
• Haemophilus influenzae
• molecular culture methods
• Staphylococcus aureus

Introduction: Conventional cultures have implicated Staphylococcus aureus (SA) and coagulase-negative Staphylococcus (CNS) as principal pathogens in chronic rhinosinusitis (CRS). These results are questioned by recent studies in which molecular probes implicate Haemophilus influenzae instead.
Objectives: To identify all bacterial species present on sinonasal mucosa using molecular culture (bacterial tag-encoded FLX amplicon pyrosequencing [bTEFAP]) and to compare them with those identified with conventional methods.
Methods: A prospective study of 18 patients undergoing endoscopic sinus surgery for CRS and 9 control patients with pituitary adenomas was conducted. Per-operative mucosal biopsies were assessed with bTEFAP by sequencing the species-specific 16S ribosomal deoxyribonucleic acid (DNA) fragment for genetic identification of bacteria and then compared with simultaneous swab culture.
Results: Standard cultures showed mainly SA and CNS. Molecular cultures identified up to 20 organisms per sample. Surprisingly, anaerobic species predominated (Diaphorobacter and Peptoniphilus). SA was nevertheless detected in 50%.
Conclusion: Molecular cultures such as bTEFAP are sensitive tools for bacterial identification in CRS and suggest that anaerobe involvement may be more frequent than presumed.

• 出版日期2010-4