Chromatin-Associated Protein Kinase C-theta Regulates an Inducible Gene Expression Program and MicroRNAs in Human T Lymphocytes

作者：Sutcliffe Elissa L; Bunting Karen L; He Yi Qing; Li Jasmine; Phetsouphanh Chansavath; Seddiki Nabila; Zafar Anjum; Hindmarsh Elizabeth J; Parish Christopher R; Kelleher Anthony D; McInnes Russell L; Taya Toshiki; Milburn Peter J; Rao Sudha^*

来源：Molecular Cell, 2011, 41(6): 704-719.

DOI：10.1016/j.molcel.2011.02.030

Studies in yeast demonstrate that signaling kinases have a surprisingly active role in the nucleus, where they tether to chromatin and modulate gene expression programs. Despite these seminal studies, the nuclear mechanism of how signaling kinases control transcription of mammalian genes is in its infancy. Here, we provide evidence for a hitherto unknown function of protein kinase C-theta (PKC-theta), which physically associates with the regulatory regions of inducible immune response genes in human T cells. Chromatin-anchored PKC-theta forms an active nuclear complex by interacting with RNA polymerase II, the histone kinase MSK-1, and the adaptor molecule 14-3-3 zeta. ChIP-on-chip reveals that PKC-theta binds to promoters and transcribed regions of genes, as well as to microRNA promoters that are crucial for cytokine regulation. Our results provide a molecular explanation for the role of PKC-theta not only in normal T cell function, but also in circumstances of its ectopic expression in cancer.

• 出版日期2011-3-18