A COMPARISON OF SILVER STAINING PROTOCOLS FOR DETECTING DNA IN POLYESTER-BACKED POLYACRYLAMIDE GEL

作者:Qiu, Shanlian; Chen, Jichen; Lin, Si; Lin, Xinjian*
来源:Brazilian Journal of Microbiology, 2012, 43(2): 649-652.
DOI:10.1590/S1517-83822012000200029

摘要

Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.

  • 出版日期2012-6
  • 单位福州大学; 福建省农业科学院

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