Porcine epidemic diarrhea virus E protein causes endoplasmic reticulum stress and up-regulates interleukin-8 expression

作者：Xu, Xingang; Zhang, Honglei; Zhang, Qi; Dong, Jie; Liang, Yabing; Huang, Yong; Liu, Hung-Jen^*; Tong, Dewen

来源：Virology Journal, 2013, 10(1): 26.

DOI：10.1186/1743-422X-10-26

• PEDV
• E protein
• ER stress
• IL-8
• NF-kb
• Bcl-2

Background: Porcine epidemic diarrhea virus (PEDV) is an important pathogen in swine and is responsible for substantial economic losses. Previous studies suggest that the PEDV E protein plays an important role in the viral assembly process. However, the subcellular localization and other functions of PEDV E protein still require more research. @@@ Methods: The subcellular localization and function of PEDV E protein were investigated by examining its effects on cell growth, cell cycle progression, interleukin-8 (IL-8) expression and cell survival. @@@ Results: The results show that plenty of PEDV E protein is localized in the ER, with small quantities localized in the nucleus. The PEDV E protein has no effect on the intestinal epithelial cells (IEC) growth, cell cycle and cyclin A expression. The cells expressing PEDV E protein express higher levels of IL-8 than control cells. Further studies show that PEDV E protein induced endoplasmic reticulum (ER) stress and activated NF-kB which is responsible for the up-regulation of IL-8 and Bcl-2 expression. @@@ Conclusions: This study shows that the PEDV E protein is localized in the ER and the nucleus and it can cause ER stress. The PEDV E protein had no effect on the IEC growth and cell cycle. In addition, the PEDV E protein is able to up-regulate IL-8 and Bcl-2 expression.

• 出版日期2013-1-19
• 单位西北农林科技大学