A prospective randomized comparison of early embryo cleavage kinetics between two media culture systems

作者：Zhang, Huan; Zheng, Yi; Wu, Yonggen; Ye, Danna; Huang, Xuefeng^*

来源：Pakistan Journal of Medical Sciences, 2016, 32(6): 1375-1379.

DOI：10.12669/pjms.326.10809

• Time-lapse imaging
• Culture media
• Embryonic development
• Morphokinetics

Objective: To investigate whether early embryo cleavage kinetics were affected by type of culture media.
Methods: In this prospective sibling-split study, 620 oocytes from 37 patients were randomly allocated into two groups: Cook group and Vitrolife group. Oocytes/embryos in Cook group, would be cultured with Cook sequential culture medium, while oocytes/embryos in Vitrolife group, would be cultured with Vitrolife sequential culture medium. Time-lapse imaging technology was used to calculate exact timing of early embryo cleavage events which included time to 2PN breakdown, cleavage to 2-, 3-, 4-, 5- cell and the time duration in the 2-,3-cell stage. Then these timing of early embryo cleavage events were compared between Cook group and Vitrolife group. Moreover, fertilization rate, cleavage rate, high quality embryo rate, usable blastocyst rate, pregnancy rate and implantation rate of these two groups were also analyzed.
Results: The results showed there were no differences in all timing of early embryo cleavage events between the two groups. In addition, the two groups were similar in fertilization rate (Cook 71.0% vs. Vitrolife 71.3%, P>0.05), cleavage rate (Cook 98.1% vs. Vitrolife 98.2%, P>0.05), high quality embryo rate (Cook 52.1% vs. Vitrolife 52.7%, P>0.05), usable blastocyst rate (Cook 29.7% vs. Vitrolife 28.0%, P>0.05), pregnancy rate (Cook 46.7% VS. Vitrolife 50.0%, P>0.05) and implantation rate (Cook 30.3% VS. Vitrolife 29.0%, P>0.05).
Conclusions: Morphokinetics used for embryo selection are not affected by the two different culture media.

• 出版日期2016-12
• 单位温州医科大学