A novel enzymatic hydrogen peroxide biosensor based on Ag/C nanocables

作者：Mao Chang Jie; Chen Xi Bao; Niu He Lin^*; Song Ji Ming; Zhang Sheng Yi; Cui Rong Jing

来源：Biosensors and Bioelectronics, 2012, 31(1): 544-547.

DOI：10.1016/j.bios.2011.10.001

• Ag/C nanocables
• Biosensor
• Horseradish peroxidase
• Hydrogen peroxide

A novel enzymatic hydrogen peroxide sensor was successfully fabricated based on the nanocomposites containing of Ag/C nanocables and gold nanoparticles (AuNPs). Ag/C nanocables have been synthesized by a hydrothermal method and then AuNPs were assembled on the surface of Ag/C nanocables. The nanocomposites were confirmed by X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometry (EDS). The above nanocomposites have satisfactory chemical stability and excellent biocompatibility. Cyclic voltammetry (CV) was used to evaluate the electrochemical performance of the Ag/C/Au nanocomposites at glassy carbon electrode (GCE). The results indicated that the Ag/C/Au nanocomposites exhibited excellent electrocatalytic activity to the reduction of H2O2. It offered a linear range of 6.7 x 10(-9) to 8.0 x 10(-6) M, with a detection limit of 2.2 x 10(-9) M. The apparent Michaelis-Menten constant of the biosensor was 51.7 x 10(-6) M. These results indicated that Ag/C/Au nanocomposites have potential for constructing of a variety of electrochemical biosensors.

• 出版日期2012-1-15
• 单位安徽大学; 常熟理工学院