Accurate Quantification of Episomal HIV-1 Two-Long Terminal Repeat Circles by Use of Optimized DNA Isolation and Droplet Digital PCR

作者：Malatinkova Eva; Kiselinova Maja; Bonczkowski Pawel; Trypsteen Wim; Messiaen Peter; Vermeire Jolien; Verhasselt Bruno; Vervisch Karen; Vandekerckhove Linos^*; De Spiegelaere Ward

来源：Journal of Clinical Microbiology, 2015, 53(2): 699-701.

DOI：10.1128/JCM.03087-14

Episomal HIV-1 two-long terminal repeat (2-LTR) circles are considered markers for ongoing viral replication. Two sample processing procedures were compared to accurately quantify 2-LTR in patients by using droplet digital PCR (ddPCR). Here, we show that plasmid isolation with a spiked non-HIV plasmid for normalization enables more accurate 2-LTR quantification than genomic DNA isolation.

• 出版日期2015-2