Antioxidant Activity of the Chemical Constituents from the Flower Buds of Magnolia denudata

作者：Seo Youngwan^*

来源：Biotechnology and Bioprocess Engineering, 2010, 15(3): 400-406.

DOI：10.1007/s12257-009-0219-4

• Magnolia denudata
• antioxidant activity
• reactive oxygen species
• ESR

The scavenging activity of the flower buds of Magnolia denudata Desrousseaux on reactive oxygen species (ROS) was evaluated using 2',7'-dichlorofluorescin diacetate (DCFH-DA) in HT 1080 cells. Methanol (MeOH) and dichloromethane (CH(2)Cl(2)) extracts inhibited dose-dependently generation of ROS in the cellular system. MeOH and CH2Cl2 extracts were combined and fractionated with n-hexane, 85% aqueous MeOH, and n-butanol (n-BuOH). Both n-hexane-soluble and 85% aqueous-soluble fractions showing strong radical-scavenging activity in the cellular system were further separated by diverse chromatographic methods to give five known lignans (1-5). All these compounds exhibited significant radical-scavenging effect on intracellular ROS in a dose-dependent manner. Their scavenging activity on various reactive oxygen species (ROS) was also evaluated using electron spin resonance (ESR) spin-trap techniques.

• 出版日期2010-6