科研之友
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摘要
Heavy metal toxicity is one of the major abiotic stresses leading to hazardous effects in plants and pollen are among the most sensitive to atmospheric pollution. Both pollen germination and tube length are inhibited by adverse environmental conditions. Pollen grains from three apple (Gala, Fuji and Braeburn) cultivars were tested for determination of pollen quality (pollen viability, germination ability and tube length) and five types of Heavy metals (Cd, Co, Pb, Hg and Zn). Viability of the pollens were determined by TTC test. The pollen germination experiments were conducted in petri dishes in 10% sucrose, 0.01% boric acid and 22 degrees C temperature for 3 hours. Among the cultivars highest pollen viability were recorded in Braeburn with 81.85%. Heavy metals lead to a significant decrease in pollen germination and tube length of apple cultivars. It was found that there were different cultivars with variable sensitivity to heavy metals on pollen germination and tube length. In general, increasing heavy metal treatments inhibited pollen germination and tube length in all cultivars. Among heavy metals mercury (Hg) had the highest toxic effect on pollen growth and tube length of all cultivars. Heavy metals have negative effect on reproduction of plant due to inhibition of pollen viability and germination.
- 出版日期2017
