科研之友
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摘要
In this paper, laser perturbation two-dimensional correlation Raman spectroscopy was explored as a rapid and reliable determination method for the assessment of bovine colostrum products and their analogue, namely, milk powder. Compared with the traditional instrumental analytical methods, such as chromatography, Raman spectroscopy measurements can be realized easily without the need of sample preparation. Two-dimensional correlation spectroscopy can enhance the spectral resolution and provide more useful information of samples. Laser perturbation technique is a new way, which has been developed in this work; additionally, only few minutes (<5 min) are in demand of the whole experiment process for one sample. Correlation coefficient method was employed to quantitatively analyze the similarities between bovine colostrum products and the other brands of milk powder that include bovine colostrum products containing some milk powder based on the developed two dimensional correlation Raman spectroscopy. The results showed that the correlation coefficients between each bovine colostrum product with their mean value could reach 0.991 +/- 0.003, which indicated that there were high similarities between these products. Under the same experimental conditions, the correlation coefficients between the bovine colostrum product (mean) and the other brands of milk powder that include bovine colostrum products containing some milk powder were located in the range of 0.596-0.761, far below the 0.991 +/- 0.003. Hence, these results clearly demonstrated the utility of the laser perturbation two-dimensional correlation Raman spectroscopy combined with correlation coefficient as a rapid method for quality control of bovine colostrum products.
