alpha-Tocopherol Prevents ERK1/2 Activation in PC12 Cells under Conditions of Oxidative Stress and Its Contribution to the Protective Effect

作者：Zakharova I O; Sokolova T V; Avrova N F^*

来源：Bulletin of Experimental Biology and Medicine, 2013, 155(1): 44-47.

DOI：10.1007/s10517-013-2076-6

• alpha-tocopherol
• PC12 cells
• ERK1/2 inhibition
• protective effect
• nanomolar concentrations

Preincubation with 100 nM and 100 mu M alpha-tocopherol for 18 h prevented long-term activation of extracellular signal-activated kinase (ERK1/2), induced by H2O2 in PC12 cells. alpha-Tocopherol significantly reduced H2O2-induced death of PC12 cell, but its protective effect was signifi cantly lower in the presence of ERK1/2 inhibitor. These data show that prevention of long-term activation of ERK1/2 by alpha-tocopherol contributes to the increase in viability of PC12 cells exposed to H2O2. This fact suggests that inhibition of ERK1/2 activity by alpha-tocopherol reduces neuronal cell death in the brain under conditions of oxidative stress in vivo.

• 出版日期2013-5