Application of Metabolic Profiling for Distinguishing Different Pathogenic Vibrio Parahaemolyticus

作者：Zhao Qiang; Sun Xiao-Hong; Lu Ying; Pan Ying-Jie; Zhao Yong^*

来源：Chemical Journal of Chinese Universities, 2012, 33(8): 1686-1691.

DOI：10.3969/j.issn.0251-0790.2012.08.010

• Vibrio parahaemolyticus
• Pathogenic
• Metabolic profiling
• Distinguish
• Potential biomarker

Distinguish of different pathogenic vibrio parahaemolyticus are normally based on tdh, trh and tlhgenes. In this research, the metabolic profiles were obtained by liquid chromatography(LC) and gas chromatography/mass spectrometry(GC-MS), and they were used for distinguishing different pathogenic vibrio parahaemolyticus. The results were compared with E. coli gene sequence between the repeat PCR (ERIC-PCR) and DNA repeats-PCR(REP-PCR). The similarity was calculated by NTsys2. 10e software. The results showed that different pathogenic Vibrio parahaemolyticus could be distinguished by LC and GC/MS. Potential biomarkers of different pathogenic strains were found by GC-MS. Strain of tdh(+), trh(-), tlh(+) have 3 kinds of potential biomarkers, strain of tdh(-), trh(+), tlh(+) have 2 kinds of potential biomarkers, strain of tdh(-), trh(-), tlh(+) have 3 kinds of potential biomarkers.

• 出版日期2012-8-10
• 单位上海海洋大学