Assessing the Validity of Fasciola hepatica ELISA Test for Immunodiagnosis of Small Ruminant Fasciolosis in Pothwar Region, Pakistan

作者：Afshan Kiran^*; Jahan Sarwat; Qayyum Mazhar

来源：Pakistan Journal of Zoology, 2017, 49(2): 737-742.

DOI：10.17582/journal.pjz/2017.49.2.sc5

• ELISA
• Immunodiagnosis
• Fasciolosis
• Ruminants
• Pakistan

To improve the diagnostic efficacy of animal fascioliasis caused by Fasciola hepatica and Fasciola gigantica in Pakistan, we first time evaluated the diagnostic accuracy of commercially available bovine F. hepatica enzyme-linked immunosorbent assay (ELISA) for the detection of IgG against fascioliasis in sera of small ruminants. The result of current study indicated diagnostic accuracy of test 95.6%, while sensitivity and specificity of this assay for small ruminants was 97.83% (95% CI: 92.35% to 99.67%) and 93.75% (95% CI: 87.54% to 97.44%) respectively. In field this test indicated significant (p < 0.05) values for sheep as compared to goat indicating high risk of infection in sheep. The current study showed that commercial bovine F. hepatica ELISA test is effective in Pakistan for small ruminants.

• 出版日期2017-4