A HOMEODOMAIN PROTEIN BINDS TO GAMMA-GLOBIN GENE REGULATORY SEQUENCES

作者：L**ELLE D^*; DUCKSWORTH J; EVES E; GOMES G; KELLER M; HELLER P; DESIMONE J

来源：Proceedings of the National Academy of Sciences of the United States of America, 1991, 88(16): 7318-7322.

DOI：10.1073/pnas.88.16.7318

Developmental regulation of gamma-globin gene expression probably occurs through developmental-stage-specific trans-acting factors able to promote the interaction of enhancer elements located in the far upstream locus control region with regulatory elements in the gamma-gene promoters and 3' A-gamma-enhancer located in close proximity to the genes. We have detected a nuclear protein in K562 and baboon fetal bone marrow nuclear extracts capable of binding to A + T-rich sequences in the locus control region, gamma-gene promoter, and 3' A-gamma-enhancer. SDS/polyacrylamide gel analysis of the purified K562 binding activity revealed a single protein of 87 kDa. A K562 cDNA clone was isolated encoding a beta-galactosidase fusion protein with a DNA binding specificity identical to that of the K562/fetal bone marrow nuclear protein. The cDNA clone encodes a homeodomain homologous to the Drosophila antennapedia protein.

• 出版日期1991-8