Diet-specific salivary gene expression and glucose oxidase activity in Spodoptera exigua (Lepidoptera Noctuidae) larvae

作者：Afshar Khashayar^*; Dufresne Philippe J; Pan Li; Merkx Jacques Magali; Bede Jacqueline C

来源：Journal of Insect Physiology, 2010, 56(12): 1798-1806.

DOI：10.1016/j.jinsphys.2010.07.012

• Gene expression
• Glucose oxidase
• Nutritional quality
• Labial saliva
• Spodoptera exigua

Saliva secreted during caterpillar feeding contains enzymes to initiate digestion or detoxify noxious plant compounds Activity of some salivary enzymes is diet-dependent and may be transcriptionally regulated In this study cDNA-amplified fragment length polymorphism was used to identify beet armyworm Spodoptera exigua Hubner labial salivary genes that are differentially expressed in response to diet In addition SeGOX was sequenced based on homology and characterized to confirm that the transcript encodes a functional enzyme Three labial salivary transcripts encoding glucose oxidase (GOX) and two proteins of unknown Se1H and Se2J) were expressed in a diet-specific manner Since diet particularly the protein to digestible carbohydrate levels and ratio may affect labial salivary enzyme activity the Influence of nutritional quality on gene expression was determined Transcript levels of the labial salivary genes Se1H Se2J and SeGOX increased with dietary carbohydrate levels regardless of protein concentrations In contrast GOX enzymatic activity Increased with increasing dietary carbohydrates when caterpillars were fed protein-rich diets but not when caterpillars were fed protein-poor diets Our results suggest that dietary carbohydrates

• 出版日期2010-12
• 单位McGill