科研之友
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摘要
The 17 beta-estradiol-imprinted polymers using non-covalent approach with methacrylic acid as the functional monomer was prepared and characterized. The effect of porogenic solvents on the adsorption capacity and thermal stability of the molecularly imprinted polymers (MIPs) were examined. Scanning electron microscopic images showed that the synthesized MIPs were bulk porous materials. The surface areas of MIPs increased from 151-188 to 239-292 m(2) g(-1) when templates were removed by methanol using Soxhlet extraction. In addition, the MIPs prepared in chloroform had a higher adsorption capacity towards 17 beta-estradiol (1,212 mu g g(-1)) than that in acetonitrile (769 mu g g(-1)), indicating that less polar porogenic solvent is suitable for synthesis of non-covalent MIPs. FTIR showed that the carbonyl group is the major functional group in MIPs to form monomer-template complex via H-bond. In addition, only a slight decrease (< 5 %) in adsorption capacity of the MIPs was observed when incubated at 80 degrees C for 5 h. Analysis of the capacity factor values (kappa(imp)') for MIPs indicated that the rebinding ability from selective recognition sites of MIPs decreased in the order 17 beta-estradiol>testosterone> benzo[a]pyrene>progesterone>phenol, and the kappa(imp)' values decreased from 2.68 to 0.63, indicating the excellent selectivity of MIPs among closely related compounds. Results obtained in this study clearly indicate that the imprinted polymer is specific for recognizing 17 beta-estradiol. The excellent selectivity and high adsorption capacity of 17 beta-estradiol-imprinted polymers open the door to develop MIPs for effective separation and adsorption of estrogenic compounds.
- 出版日期2012-8
- 单位清华大学
