Anti-inflammatory Activity of Magnesium Isoglycyrrhizinate Through Inhibition of Phospholipase A2/Arachidonic Acid Pathway

作者：Xie Chunfeng; Li Xiaoting; Wu Jieshu; Liang Zhaofeng; Deng Feifei; Xie Wei; Zhu Mingming; Zhu Jianyun; Zhu Weiwei; Geng Shanshan; Zhong Caiyun^*

来源：Inflammation, 2015, 38(4): 1639-1648.

DOI：10.1007/s10753-015-0140-2

• magnesium isoglycyrrhizinate
• inhibition
• phospholipase A(2)
• arachidonic acid
• inflammatory lipid mediators

Glycyrrhiza glabra (licorice) has been known to possess various pharmacological properties including anti-inflammatory, antioxidants, antiviral, and hepatoprotective activities. Magnesium isoglycyrrhizinate (MgIG), a magnesium salt of 18-alpha glycyrrhizic acid stereoisomer, is clinically used for the treatment of inflammatory liver diseases. However, the mechanism by which MgIG exerts its anti-inflammatory effects remains unknown. In the present study, we investigated the inhibitory potential of MgIG in phospholipase A(2) (PLA(2))/arachidonic acid (AA) pathway and release of the pathway-generated inflammatory lipid mediators in RAW264.7 macrophages. Results revealed that MgIG suppressed LPS-induced activation of PLA(2) and production of AA metabolites such as prostaglandin E-2 (PGE(2)), prostacyclin (PGI(2)), thromboxane 2 (TXB2), and leukotrienes (LTB4) in macrophages. Furthermore, LPS-induced AA-metabolizing enzymes including COX-2, COX-1, 5-LOX, TXB synthase, and PGI(2) synthase were significantly inhibited by MgIG. Taken together, our data suggest that modulation of cyclooxygenase (COXs) and 5-lipoxygenase (LOX) pathways in AA metabolism could be a novel mechanism for the anti-inflammatory effects of MgIG.

• 出版日期2015-8
• 单位南京医科大学