Decreased 1,25-Dihydroxyvitamin D3 level is involved in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease

作者：Yi, Xianglong; Yang, Peizeng^*; Sun, Min; Yang, Yan; Li, Fuzhen

来源：Molecular Vision, 2011, 17(77-78): 673-679.

• EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS
• VITAMIN-D
• T-CELLS
• D-3
• EXPRESSION
• 25-HYDROXYVITAMIN-D
• PERSPECTIVES
• RECEPTORS
• PATTERNS
• ANALOGS

Purpose: 1,25-Dihydroxyvitamin D-3 [1,25(OH)(2)D-3] has recently been found to be involved in the development of autoimmune diseases. This study was to investigate the expression and potential role of 1,25(OH)(2)D-3 in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease. @@@ Methods: Blood samples were obtained from VKH patients and healthy individuals. Serum 1,25(OH)(2)D-3 levels were measured using ELISA. Peripheral blood mononuclear cells (PBMCs) or cluster of differentiation (CD) 4(+) T cells were cultured with or without 1,25(OH)(2)D-3 in the presence of anti-CD3 and anti-CD28 for the measurement of cell proliferation and cytokines. The cell proliferation was detected using the Cell Counting Kit. The levels of interleukin (IL)-17 and interferon (IFN)-gamma levels in the supernatants of PBMCs or CD4(+) T cells were detected by ELISA. @@@ Results: 1,25(OH)(2)D-3 was significantly decreased in the serum of active VKH patients as compared with inactive VKH patients and controls. It significantly inhibited PBMCs proliferation and CD4(+) T cell proliferation. It was also able to significantly inhibit the production of IL-17 and IFN-gamma by both PBMCs and CD4(+) T cells from VKH patients and controls. @@@ Conclusions: These findings suggest that decreased expression of 1,25(OH)(2)D-3 may be involved in the development of VKH disease. 1,25(OH)(2)D-3 may be potentially used in the treatment of this disease.

• 出版日期2011-3-9
• 单位中山大学; 重庆医科大学