A molecular signature of an arrest of descent in human parturition

作者：Mittal Pooja^*; Romero Roberto; Tarca Adi L; Draghici Sorin; Nhan Chang Chia Ling; Chaiworapongsa Tinnakorn; Hotra John; Gomez Ricardo; Kusanovic Juan Pedro; Lee Deug Chan; Kim Chong Jai; Hassan Sonia S

来源：American Journal of Obstetrics and Gynecology, 2011, 204(2): 177.e15.

DOI：10.1016/j.ajog.2010.09.025

• adenosine triphosphatase
• Na plus /K plus transporting
• alpha 1 polypeptide
• ATP1A1
• COX2
• HIF1A
• hypoxia inducible factor-1
• inflammation
• interleukin-6
• myometrium
• parturition
• pregnancy
• prostaglandin-endoperoxide synthase 2
• PTGS2
• spontaneous term labor
• systems biology
• transcriptomics

OBJECTIVE: This study was undertaken to identify the molecular basis of an arrest of descent.
STUDY DESIGN: Human myometrium was obtained from women in term labor (TL; n = 29) and arrest of descent (AODes; n = 21). Gene expression was characterized using Illumina HumanHT-12 microarrays. A moderated Student t test and false discovery rate adjustment were applied for analysis. Confirmatory quantitative reverse transcription-polymerase chain reaction and immunoblot were performed in an independent sample set.
RESULTS: Four hundred genes were differentially expressed between women with an AODes compared with those with TL. Gene Ontology analysis indicated enrichment of biological processes and molecular functions related to inflammation and muscle function. Impacted pathways included inflammation and the actin cytoskeleton. Overexpression of hypoxia inducible factor-1a, interleukin -6, and prostaglandin-endoperoxide synthase 2 in AODes was confirmed.
CONCLUSION: We have identified a stereotypic pattern of gene expression in the myometrium of women with an arrest of descent. This represents the first study examining the molecular basis of an arrest of descent using a genome-wide approach.

• 出版日期2011-2