Dose-dependent Cytotoxicity and Oxidative Stress Induced by "Naked" Fe3O4 Nanoparticles in Human Hepatocyte

作者：Lin Xin li; Zhao Shu hua; Zhang Long; Hu Gui qin; Sun Zhi wei^*; Yang Wen sheng

来源：Chemical Research in Chinese Universities, 2012, 28(1): 114-118.

• Superparamagnetic
• Fe3O4
• Cytotoxicity
• Oxidative stress
• DNA damage
• Cell cycle
• Cell apoptosis

The dose-dependent cytotoxicity effect on human hepatocyte(HL-7702 cells) induced by "naked" Fe3O4 nanoparticles was assessed through cell viabilities and lactate dehydrogenase(LDH) activities. Three important oxidative indexes of the cells by glutathione peroxidase(GSH-Px), superoxide dismutase(SOD) and malondialdehyde(MDA) were determined. The good correlation of the cell viabilities with their GSH-Px, SOD and MDA levels indicated that the cytotoxicity is related to activation of oxidative stress induced by Fe3O4 nanoparticles. The oxidative stress also leads to corresponding DNA damage in a similar dose-dependent manner, followed by the changes of cell cycle and cell apoptosis. Such work provides important experimental data for the safety evaluation of superparamagnetic Fe3O4 nanoparticles.

• 出版日期2012-1-25
• 单位吉林大学; 首都医科大学