The orphan G protein-coupled receptor 161 is required for left-right patterning

作者：Leung TinChung^*; Humbert Jasper E; Stauffer Anna M; Giger Kathryn E; Chen Hui; Tsai Huai Jen; Wang Chuan; Mirshahi Tooraj; Robishaw Janet D

来源：Developmental Biology, 2008, 323(1): 31-40.

DOI：10.1016/j.ydbio.2008.08.001

• Orphan GPCR
• Ca2+
• Left-right asymmetry
• Cardiac looping
• Visceral asymmetry
• Zebrafish development

Gpr161 (also known as RE2) is an orphan G protein-coupled receptor (GPCR) that is expressed during embryonic development in zebrafish. Determining its biological function has proven difficult due to lack of knowledge regarding its natural or synthetic ligands. Here, we show that targeted knockdown of gpr161 disrupts asymmetric gene expression in the lateral plate mesoderm, resulting in aberrant looping of the heart tube. This is associated with elevated Ca2+ levels in cells lining the Kupffer's vesicle and normalization of Ca2+ levels, by over-expression of ncx1 or pmca-RNA, is able to partially rescue the cardiac looping defect in gpr-161 knockdown embryos. Taken together, these data support a model in which gPr161 plays an essential role in left-right (L-R) patterning by modulating Ca2+ levels in the cells surrounding the Kupffer's vesicle.

• 出版日期2008-11-1