科研之友
微信
新浪微博
Facebook
分享链接
摘要
A soybean cDNA clone, 15-9-A, corresponding to a new nodule-specific gene was isolated. 15-9-A corresponds to an abundantly transcribed 1.0-kb mRNA that could first be detected in root nodules at about 10 days following inoculation with Bradyrhizobium japonicum. This clone was found to be very closely related to, but distinct from, Ngm-20, a member of a soybean nodulin multigene family. We have used oligonucleotide probes to detect an additional closely related gene, Ngm-20r, which is expressed as a 0.8-kb mRNA. 15-9-A, Ngm-20, and Ngm-20r appear to form a subfamily within the larger nodulin multigene family. Results derived from sequence analysis and hybridization experiments suggest that gene conversion may have played a role in the evolution of this subfamily.
- 出版日期1991-12
