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摘要
In order to study anti-complement activity in the classical pathway of Korean Mondshood root and its sulfated polysaccharides, a homogeneous polysaccharide(KMPS-2A) was gained from the Korean Mondshood root[ Aconitum, coreanum, Levl.) Raipaics] by water extract and alcohol precipitate via DE-32, Superdex-200 and Superdex-75 gel filtration. Its structure was investigated by high performance gel permeation chromatography, methylation analysis, NMR and IR spectrometry. The results showed that the average molecular weights (M-w) of the KMPS-2A was 676000 and it was consisted of a repeating structure as [alpha-1,6-D-Glc](n). Sulfated derivatives were gained by chlorosulfonic acid-pyridine method and it was found that the sulfated derivatives had the highest degree of substitution (1.79) when the volume ratio of chlorosulfonic acid and pyridine was 1.75 : 1.0. It was shown that sulfated polysaccharide 1.75B had a better anti-complementary activity. C2, C3 and C4 were substituted by sulfate groups. A certain correlation was presented between the degree of substitution and the anticomplementary activity of sulfated derivatives. Anticomplementary activity of sulfated derivative 1.75B was better than the heparin group in the classical pathway, which suggested the potential activity of the complement inhibitors.
- 出版日期2014-7-10
- 单位上海中药标准化研究中心; 上海中医药大学
