科研之友
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摘要
A tolerance trial was carried out to evaluate the safety of feeding multifold doses of a model flavour containing a mixture of 20 chemically defined flavouring compounds to crossbred weanling piglets (Duroc male x Landrace/Large White female). Animals were weaned at 28 days and assigned to one of five treatments: a control (no flavour) or diets containing the model flavour at levels of 1x, 3x, 10x or 30x the typical commercial inclusion rate of 500 mg/kg feed. A pre-starter diet was fed to piglets from 28 to 42 days of age and a starter diet from 42 to 70 days of age. Parameters measured included average daily gain (ADG), average daily feed intake (ADFI), feed conversion ratio FCR), haematology, serum biochemistry, general health and mortality. The model flavour was well tolerated by the piglets at all four dosage levels. No differences in ADFI or FCR were observed between treatment groups during the pre-starter/starter periods or for the whole trial. The ADG of piglets in receiving 3x the normal dosage was less (P<0.05) than that of the control animals, but not different from the other dosage groups. The group of piglets with the lower ADG had a higher incidence of diarrhoea than the other groups. Differences observed among treatments for the haematology and serum biochemistry parameters analysed were limited to piglets fed 3x the normal dosage. The extraction method developed to quantify the different compounds of the model flavour using routine GC/MS RTL is described. The importance of monitoring flavour content of the constituents present in the mixture and reducing losses due to volatility is highlighted. Results obtained from the trial demonstrate the feasibility of tolerance testing in target animal species using flavour mixtures.
- 出版日期2016-6
