Differential axial contrast of optical sections: laser microtomography and quantitative 3D reconstruction

作者：Pogorelova M A^*; Golichenkov V A; Pogorelov A G

来源：Optics and Spectroscopy, 2014, 116(3): 488-493.

DOI：10.1134/S0030400X14030175

Specific features of the quantitative laser microtomography of biological samples are discussed. The method exhibits the main advantages of a confocal microscope (rapid measurement of a stack of parallel optical cross sections and accurate displacement of an object along the optical axis). A relatively high contrast is reached owing to the superposition of pairwise complementary images on neighboring cross sections. A simple and convenient algorithm for image processing does not require additional software and can be computerized using a conventional graphic editor. The applicability of the method is illustrated using volume measurements of a single cell of an early mouse embryo.

• 出版日期2014-3