Nucleases and helicases take center stage in homologous recombination

作者：Mimitou Eleni P; Symington Lorraine S^*

来源：TRENDS IN BIOCHEMICAL SCIENCES, 2009, 34(5): 264-272.

DOI：10.1016/j.tibs.2009.01.010

Homologous recombination (HR)-mediated DNA double-strand break repair maintains genome integrity. Although long-studied, an understanding of two essential steps in this process - the resection of DNA ends to produce recombinogenic 3' single-stranded DNA tails and the resolution of recombination intermediates-has remained elusive. Recent findings show an unexpected role for the Sgs1 (BLM) helicase and Dna2 nuclease in end resection, and provide mechanistic insight into the initiation of 5'-3' resection as well as its regulation by the cell cycle and the DNA damage response. Moreover, the identification of a novel Holliday junction resolvase, Yen1 (GENU and several helicases that dismantle strand invasion intermediates has increased the repertoire of nucleases and helicases capable of resolving recombination intermediates.

• 出版日期2009-5