Diversification of IFN gamma-inducible CXCb chemokines in cyprinid fish

作者：van der Aa Lieke M; Chadzinska Magdalena; Derks Wouter; Scheer Marleen; Levraud Jean Pierre; Boudinot Pierre; Verburg van Kemenade B M Lidy^*

来源：Developmental and Comparative Immunology, 2012, 38(2): 243-253.

DOI：10.1016/j.dci.2012.05.005

• CXCb chemokines
• Cyprinids
• Promoter analysis
• Leukocyte stimulation

We earlier identified two CXCL8-like lineages in cyprinid fish, which are functional homologues of the mammalian CXCL8, but with diverged functions. We here investigated whether the carp IFN-gamma-inducible CXCb gene, related to the mammalian CXCL9, -10 and -11 chemokines, was subject to a similar diversification. On the zebrafish genome, a cluster of seven CXCb genes was found on chromosome five. Analysis of the promoter of the zebrafish CXCb genes suggests a partially shared, but differential induction. A second CXCb gene, CXCb2, was identified in common carp by homology cloning. CXCb2 is constitutively expressed in immune-related tissues, predominantly in head kidney lymphocytes/monocytes. Interestingly, an induction of CXCb2 gene expression with recombinant carp IFN-gamma 2 and LPS was observed in macrophages and granulocytes. Finally, difference in sensitivity to LPS, and kinetics of CXCb1 and CXCb2 gene expression during zymosan-induced peritonitis, was observed. These results indicate a functional diversification for cyprinid CXCb chemokines, with functional homology to mammalian CXCL9-11.

• 出版日期2012-10