Antioxidant Opuntia ficus-indica Extract Activates AHR-NRF2 Signaling and Upregulates Filaggrin and Loricrin Expression in Human Keratinocytes

作者：Nakahara Takeshi; Mitoma Chikage; Hashimoto Hachiya Akiko; Takahara Masakazu; Tsuji Gaku; Uchi Hiroshi; Yan Xianghong; Hachisuka Junichi; Chiba Takahito; Esaki Hitokazu; Kido Nakahara Makiko; Furue Masutaka^*

来源：Journal of Medicinal Food, 2015, 18(10): 1143-1149.

DOI：10.1089/jmf.2014.3396

• < bold> antioxidant< /bold> < bold> aryl hydrocarbon receptor< /bold> < bold> filaggrin< /bold> < bold> Opuntia ficus-indica< /bold> < bold> extract< /bold> < bold> NRF2< /bold> < bold> NQO1< /bold&gt

Opuntia ficus-indica (OFI) is a cactus species widely used as an anti-inflammatory, antilipidemic, and hypoglycemic agent. It has been shown that OFI extract (OFIE) inhibits oxidative stress in animal models of diabetes and hepatic disease; however, its antioxidant mechanism remains largely unknown. In this study, we demonstrated that OFIE exhibited potent antioxidant activity through the activation of nuclear factor erythroid 2-related factor 2 (NRF2) and the downstream antioxidant enzyme NAD(P)H:quinone oxidoreductase 1 (NQO1), which inhibited the generation of reactive oxygen species in keratinocytes challenged with tumor necrosis factor or benzo[]pyrene. The antioxidant capacity of OFIE was canceled in NRF2 knockdown keratinocytes. OFIE exerted this NRF2-NQO1 upregulation through activation of the aryl hydrocarbon receptor (AHR). Moreover, the ligation of AHR by OFIE upregulated the expression of epidermal barrier proteins: filaggrin and loricrin. OFIE also prevented TH2 cytokine-mediated downregulation of filaggrin and loricrin expression in an AHR-dependent manner because it was canceled in AHR knockdown keratinocytes. Antioxidant OFIE is a potent activator of AHR-NRF2-NQO1 signaling and may be beneficial in treating barrier-disrupted skin disorders.

• 出版日期2015-10-1