New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR

作者：Rogawski Rivkah; McDermott Ann E

来源：Archives of Biochemistry and Biophysics, 2017, 628: 102-113.

DOI：10.1016/j.abb.2017.06.010

• Dynamic nuclear polarization
• Solid state NMR
• Nitroxide biradical
• Paramagnetic tags
• In cell NMR

Magic angle spinning solid state NMR studies of biological macromolecules [1-3] have enabled exciting studies of membrane proteins [4,5], amyloid fibrils [6], viruses, and large macromolecular assemblies [7]. Dynamic nuclear polarization (DNP) provides a means to enhance detection sensitivity for NMR, particularly for solid state NMR, with many recent biological applications and considerable contemporary efforts towards elaboration and optimization of the DNP experiment. This review explores precedents and innovations in biological DNP experiments, especially highlighting novel chemical biology approaches to introduce the radicals that serve as a source of polarization in DNP experiments.

• 出版日期2017-8-15