A novel synthetic peptide from a tomato defensin exhibits antibacterial activities against Helicobacter pylori

作者：Rigano M M; Romanelli A; Fulgione A; Nocerino N; D' Agostino N; Avitabile C; Frusciante L; Barone A; Capuano F; Capparelli R^*

来源：Journal of Peptide Science, 2012, 18(12): 755-762.

DOI：10.1002/psc.2462

• antimicrobial peptide
• defensin
• tomato
• Helicobacter pylori

Defensins are a class of cysteine-rich proteins, which exert broad spectrum antimicrobial activity. In this work, we used a bioinformatic approach to identify putative defensins in the tomato genome. Fifteen proteins had a mature peptide that includes the well-conserved tetradisulfide array. We selected a representative member of the tomato defensin family; we chemically synthesized its gamma-motif and tested its antimicrobial activity. Here, we demonstrate that the synthetic peptide exhibits potent antibacterial activity against Gram-positive bacteria, such as Staphylococcus aureus A170, Staphylococcus epidermidis, and Listeria monocytogenes, and Gram-negative bacteria, including Salmonella enterica serovar Paratyphi, Escherichia coli, and Helicobacter pylori. In addition, the synthetic peptide shows minimal (%26lt;5%) hemolytic activity and absence of cytotoxic effects against THP-1 cells. Finally, SolyC exerts an anti-inflammatory activity in vitro, as it downregulates the level of the proinflammatory cytokines TNF-alpha and IFN-gamma.

• 出版日期2012-12