Differential expression of ACINUS variants in bone marrow in myelodysplastic syndromes

作者：Miyamoto Norikazu; Kurata Morito; Nagiri Toshiya; Abe Shinya; Yamamoto Kouhei; Onishi Iichiroh; Kirimura Susumu; Murayama Toshihiko; Hidaka Michihiro; Kitagawa Masanobu^*

来源：International Journal of Clinical and Experimental Pathology, 2017, 10(4): 4330-4338.

• MDS
• AML
• ACINUS
• cell proliferation
• apoptosis

Hematopoietic cells in the bone marrow of myelodysplastic syndromes (MDS) exhibit a complex pattern of cellular dynamics, showing active cellproliferation, as well as frequent apoptosis. To determine the influence of ACINUS expression on the regulation of cellular dynamics in MDS, the expression of ACINUS in bone marrow samples from controls and patients with MDS, overt leukemia (OL) transformed from MDS and de novo acute myeloid leukemia (AML) was analyzed using immunohistochemistry and real-time quantitative PCR (RT-PCR). The expression of the ACINUS protein was up-regulated in the bone marrow of MDS, OL, and de novo AML. RNA of ACINUS-S' also up-regulated as the same pattern, whereas ACINUS-L did not. In vitro experiments demonstrated that overexpression of ACINUS-S' resulted in increased cell proliferation. These results suggest that ACINUS variants exhibit differential patterns of expression and function in the bone marrow of myeloid neoplasms, such as MDS, OL, and de novo AML.

• 出版日期2017