DEAD Box Protein DDX1 Regulates Cytoplasmic Localization of KSRP

作者：Chou Chu Fang; Lin Wei Jye; Lin Chen Chung; Luber Christian A; Godbout Roseline; Mann Matthias; Chen Ching Yi^*

来源：PLos One, 2013, 8(9): e73752.

DOI：10.1371/journal.pone.0073752

mRNA decay mediated by the AU-rich elements (AREs) is one of the most studied post-transcriptional mechanisms and is modulated by ARE-binding proteins (ARE-BPs). To understand the regulation of K homology splicing regulatory protein (KSRP), a decay-promoting ARE-BP, we purified KSRP protein complexes and identified an RNA helicase, DDX1. We showed that down-regulation of DDX1 expression elevated cytoplasmic levels of KSRP and facilitated ARE-mediated mRNA decay. Association of KSRP with 14-3-3 proteins, that are predominately located in the cytoplasm, increased upon reduction of DDX1. We also demonstrated that KSRP associated with DDX1 or 14-3-3, but not both. These observations indicate that subcellular localization of KSRP is regulated by competing interactions with DDX1 or 14-3-3.

• 出版日期2013-9-4