Adjuvant effect of Shilajit and plant extracts on the immune responses to bovine serum albumin in hens

作者：Bizanov Gazim^*; Barinova Natalja; Jonauskiene Irena

来源：Central European Journal of Immunology, 2012, 37(2): 91-95.

• Shilajit
• Hedysarum neglectum
• Ginkgo biloba
• hens
• oral administration
• mucosal immunity.

The aim of current study was to test an immunostimulalor activity of three different components such as Shilajit. Hedysarum neglectum and Ginkgo biloba that correspond to A, B and C groups respectively Three groups of hens were immunized orally with a bovine serum albumin (BSA) three times once a week. Serum and saliva were collected every 7th day during 9 weeks. Eggs were taken every day throughout the experiment. BSA-specific immunoglobulin A (IgA) antibodies in saliva, IgM antibodies in serum and IgY antibodies in egg yolk were examined by ELISA. A total IgY (mg/ml) was measured using a photometric technique. We observed an immense increase of IgA titres in two groups that were immunized with Shilajit and H. neglectum. Immunoglobulin Y litres were higher in the group A. However the total IgY is mounted four limes more in the group B. Immunoglobulin M titres were at the similar level throughout the observation period. Interestingly, G. biloba showed the poorest Ig titres. Summarizing the named herbomineral and two medicinal plants can be advised to boost the immune response in birds.

• 出版日期2012