科研之友
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摘要
Biochips with amino groups on the substrate surface were prepared through a special immersion method using absolute ethanol as the solvent, 3-aminopropyltriethoxysilane (APTES) as the modifying agent and acetic acid as the catalyst. The impacts of different process parameters in the preparation process on the substrate noise were mainly studied, including soaking time, APTES concentration, acid treatment time and hydroxylation time. Through stage-by-stage optimization of the process parameters above, the substrate noise decreased effectively, with the minimum value of 193 obtained. The results of the spotting test and hybridization test of the substrates pre-treated under the optimum modification condition show that the substrate prepared by this method could effectively connect the gene probe, with the hybridization signal intensity after cleaning up to 17 000 and the signal-to-noise ratio above 110. The X-ray photoelectron spectroscopy (XPS) analysis indicates a successful integration of amino group onto the surface of biochip substrate after modification. It was found that the modification process had little effect on the transmittance of substrates, all kept above 91%, and thus ensured the accuracy of the hybridization signal and microarray noise detection followed.
- 出版日期2012-5-10
- 单位华东理工大学
