Detection of growth factor binding to gelatin and heparin using a photonic crystal optical biosensor

作者：Morgan Abby W; Chan Leo L^*; Sendemir Urkmez Aylin; Cunningham Brian T; Jamison Russell D

来源：Materials Science & Engineering C-Materials for Biological Applications, 2010, 30(5): 686-690.

DOI：10.1016/j.msec.2010.02.025

• Photonic crystal biosensor
• Label-free
• Gelatin
• Heparin
• Growth factor
• Drug-carrier

Drug-carrier interactions are important to protein controlled release systems to protect the protein from denaturation and ensure properly timed release. A novel photonic crystal biosensor was used to investigate a gelatin-protein controlled release system to determine the amount of protein bound to the carrier at physiological conditions. The Biomolecular Interaction Detection (BIND) system reflects a narrow band of wavelengths when white light is shone incident to the grating. As mass is deposited onto the surface, the peak wavelength value is shifted due to changes in the optical density of the biosensor. The BIND system was used to detect the binding of growth factors onto acidic gelatin, basic gelatin, and heparin on the sensor surface. Through a series of experiments, including functionalizing the sensor, adjusting the ionic strength of the solution, adjusting the substrate concentration, and minimizing non-specific signal, the adsorption of the gelatins and heparin on the sensor was enhanced. The binding interaction of recombinant human transforming growth factor (rhTGF)-beta 1 and bone morphogenetic protein (rhBMP)-2 with the two types of gelatin and heparin were investigated. The strength of the interaction between rhTGE-beta 1 and the substrates is in the following order: heparin > acidic gelatin > basic gelatin. RhBMP-2 bound to the substrates but with less intensity than TGF-beta 1: heparin > basic gelatin > acidic gelatin. This work provides support for the controlled release mechanism through degradation of the gelatin carrier.

• 出版日期2010-6-15
• 单位美国弗吉尼亚理工大学(Virginia Tech)