Application of the Nanogold-4,4 '-bis(methanethiol)biphenyl modified gold electrode to the determination of tyrosinase-catechol reaction kinetics in acetonitrile

作者：Nakamura Toshio^*; Ren Jujie; Zhu Kai mei; Kawara Shinshi; Jin Baokang

来源：Analytical Sciences, 2006, 22(9): 1261-1264.

DOI：10.2116/analsci.22.1261

The reactivity of tyrosinase adsorbed on nanogold bound with 4,4'-bis(methanethiol)biphenyl monolayer self-assembled on a gold disk with catechol in a dipolar aprotic solvent, acetonitrile (AN), was studied by cyclic voltammetric and amperometric methods. Tyrosinase exhibited characteristics of a Michaelis-Menten kinetic mechanism. The tyrosinase attached to the nanogold continued to react with substrates in AN even when the water content was lower than 0.01 w/w%. The apparent Michaelis-Menten constant K-m of tyrosinase for catechol is 5.5 +/- 0.4 mM (n = 5).

• 出版日期2006-9