Autologous fat injection therapy including a high concentration of adipose-derived regenerative cells in a vocal fold paralysis model: animal pilot study

作者：Nishio N^*; Fujimoto Y; Suga K; Iwata Y; Toriyama K; Takanari K; Kamei Y; Yamamoto T; Gotoh M

来源：Journal of Laryngology and Otology, 2016, 130(10): 914-922.

DOI：10.1017/S0022215116008707

• Stem Cells
• Injections
• Vocal Cord Paralysis
• Animal
• Hoarseness

Objectives: To verify the effectiveness and safety of the addition of adipose-derived regenerative cells to autologous fat injection therapy.
Methods: Unilateral vocal fold paralysis models were made by cutting the right recurrent laryngeal nerve in two pigs. At day 30, 0.5 ml adipose-derived regenerative cells mixed with 1 ml autologous fat was injected into the right vocal fold of one pig, with the other receiving 0.5 ml Ringer's solution mixed with 1 ml autologous fat. At day 120, fibrescopy, laser Doppler flowmeter, computed tomography, vocal function evaluation and histological assessment were conducted.
Results: Although histological assessment revealed atrophy of the thyroarytenoid muscle fibre in both pigs, there was remarkable hypertrophy of the thyroarytenoid muscle fibre in the area surrounding the adipose-derived regenerative cells injection site.
Conclusion: The addition of a high concentration of adipose-derived regenerative cells to autologous fat injection therapy has the potential to improve the treatment outcome for unilateral vocal fold paralysis.

• 出版日期2016-10